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| Studies on Preparation,Characteristic Appraisal and Application of Human Cardiac Myosin and Human Cardiac Myosin Heavy Chain |
| SONG Na-ling, ZHAO Qi-ren, LIU Jia-hui, et al |
| The key laboratory of molecular nuclear medicine, institute of radiation medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192 China |
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Abstract Objective To isolate, extract and purify human cardiac myosin (HCM) and human cardiac myosin heavy chain (HCMHC) for it's next study of myocardid image and the antibody against HCMHC imaging reagent.Methods First extract HCM from human heart muscle tissue and prepare HCMHC from the HCM, detecte characteristics including protein concentration, molecular weight, enzymatic activity and free SH groups of the HCM and the HCMHC, prepare hybridoma cell lines of HCMHC, and examine the immunology activity of the HCMHC.Results 24mg HCM were extracted from 127g human cardiac muscle tissue, and 9.1mg HCMHC were prepared.Elecrtrophoresis of SDS-PAGE (gradient) showed that the band of HCMHC located at 200 kD, which was the same of that of literatures, purity of HCMHC is higher than 80%.The specific activity of ATPase of HCM is good.The highest titer of anti-HCMHC serum is about 107.The fusion rate was 80%.Conclusions HCMHC has good immunology activity, for it's next study of the antibody against HCMHC single chain fragment variable (ScFv) radionuclide receptor imaging.
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Received: 02 April 2007
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SONG Na-ling, ZHAO Qi-ren, ZHANG Chun-ming, LIU Jia-hui, LIU Jie, CHU Li-ping, WANG De-zhi, HE Xin, WANG Yue-ying. Synthesiaed Genes of VH and VL of Single-Chain Antibody of Human Cardiac Myosin Heavy Chain[J]. , 2012, 21(1): 1-3. |
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