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| Prediction of Radiosensitivity of Human Tumor Cell Lines in Vitro by Determining 4977bp Deletion in Mitochondrial DNA |
| RONG Qing-lin1, ZHAO Xin-ran2, WANG Qin2, et al |
| Department of Radiation Oncology, General Hospital of Tianjin Medical University, Tianjin 300052 China |
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Abstract Objective To evaluate the possibility of predicting the radiosensitivity of tumor cell lines using the assay of the mtDNA4977bp deletion.Methods The mtDNA4977bp deletion of HepG2 cells and PC-3 cells were detected by nested PCR after irradiated by various doses of x-ray.Results The radiation-induced mtDNA4977bp deletion of the tumor cell lines of HepG2 and PC-3 were detected after irradiated.There was a dose dependent in the mtDNA4977bp deletion of two tumor cell lines.The deletion rate of HepG2 was higher significantly than that of PC-3 at each point of radiation dose (P〈0.05), which implies that the radiosensitivity of HepG2 was higher than that of PC-3.Conclusion The assay of the mtDNA4977bp deletion may be an approach to predict the radiosensitivity of tumor cells.
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Received: 12 May 2008
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