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| The Study on the Items of Oxidation and Anti-oxidation in the Rats with Radiation Esophagitis Treated with Compound White Peony Root Oral Liquids |
| SHEN Li1, SHAN Bao-en2, ZHANG Li2, et al |
| The First Hospital of Hebei Medical University, Shijiazhuang 050011 China |
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Abstract Objective To study the content of the hydroxyl radical (OH·)and NO, the activity of CAT and GSH-PX in rats' esophagus and NO level in serum from the Wistar rats radiated for esophagitis area treated with compound white peony root oral liquids (cWPROL).Methods 96 Wistar rats were divided into eight groups.Group 1, the normal group, were without any radiation and treatment;Group 2, radiation group 1, killed on the seventh day after being radiated by 43Gy 60Co-γ ray;Group 3, radiated group 2, killed on the fifteenth day after being radiated;Group 4 given cWPROL (normal dose)for prevention at the same time of radiation;Group 5 given cWPROL (high dose)for prevention at the same time of radiation;Group 6 were treated with cWPROL (normal dose)from the seventh day after radiation;Group 7 were treated with cWPROL (high dose)from the seventh day after radiation;Group 8 were treated with Western medicine from the seventh day after radiation.On every experimental finished point, the blood serum and full-length esophagus of rats were kept for examination.The content of hydroxyl radical (OH·)and NO, and the activity of CAT and GSH-PX in rats' esophagus tissues were determined.The content of NO in rats' blood serum was also analyzed.Results The content of hydroxyl radical (OH·)and NO and in Wistar rats' esophagus tissue increased significantly compared with that of the normal rat on the 14th day radiated by 43Gy 60Co-γ (P<0.05).The activity of CAT and GSH-PX increased significantly (P<0.01).However, the content of hydroxyl radical (OH·)and NO, and the activity of CAT and GSH-PX in the esophagus tissue of rat treated with cWPROL decreased significantly compared with that of the rat in radiated group 2 (P<0.05), the rat treated with cWPROL of high dose from the seventh day after radiation (P<0.01).The content of NO in blood serum was no significantly different among all of the groups (P>0.05).The content of NO and the activity of CAT and GSH-PX in the esophagus tissue were reduced in the rats treated with Western medicine (P<0.05)compared with that of the rats in radiation group 2, especially the marked decrease in the content of NO (P<0.01).Conclusion cWPROL can reduce the content of hydroxyl radical (OH·)and NO the activity of GSH-PX and CAT in esophagus tissue of rat radiated with 60Co-γ ray, which maybe is the mechanism for the treatment of inflammatory esophagus induced by radiation.
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Received: 30 October 2007
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