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| The Effects of Electromagnetic Irradiation on Activation of Microglia and JAKs in Rat Hippocampus |
| CHEN Chun-hai, YANG Xue-sen, HAO Yu-tong, et al |
| The Department of Occupational Hygiene, the Third Military Medical University, Chongqing 400038 China |
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Abstract Objective To determine the activation of microglia and the phosphorylation of Jaks, the upstream factors of JAK/STAT (janus activated kinase/signal transducers and activators of transcription)signaling pathway, after electromagnetic irradiation.Methods Rats were irradiated by 90 mW/cm2 EMF for 20 min.The phosphorylation of Jaks was determined by western blot at different time after electromagnetic irradiation.The activation of microglia was determined by immunochemistry.Results GSA-IB4 was upregulated in microglia, which indicated microglia was activated after electromagnetic irradiation.The phosphorylation of Jak1, Jak2 and Jak3 in rat hippocampus was upregulated after electromagnetic irradiation.The phosphorylation of Jak1 was upregulated after microwave exposure and peaked at 12 h.Jak2 peaked at 0 h after electromagnetic irradiation and sustained in a high level.Jak3 was slightly affected by electromagnetic irradiation.All the three members of JAKs return to normal at 72 h after electromagnetic irradiation.Conclusion Microglia cells was activated after electromagnetic irradiation.The phosphorylation of Jaks was upregulated by electromagnetic irradiation.It suggested that JAK/STAT singnaling pathway was activated after electromagnetic irradiation, which indicated that JAK/STAT signaling pathway may participate in brain microglia activation induced by electromagnetic irradiation.
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Received: 03 September 2007
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