|
|
|
| Adaptive Response of EL-4 Lymphoma Cell Cycle Progression Induced by Low Dose Radiation in Vitro |
| LIU Shu-chun, WANG Zhi-cheng, LIU Yang, et al |
| Key Laboratory of Radiobiology, Ministry of Health(School of Public Health, Jilin University), Changchun 130021 China |
|
|
|
|
Abstract Objective To explore the adaptive response of EL-4 cycle progression induced by low dose radiation in vitro.Methods EL-4 lymphoma cells were irradiated with the inductive doses (D1:25-200 mGy, 12.5 mGy/min)and the challenging dose (D2:0.5-3.0 Gy, 287 mGy/min).The interval time between D1 and D2 was 6 h.The cell percentages of each cell cycle phase were measured with flow cytometry.Results The cell percentages of G0/G1 phase in the D1 + D2 group with25-100 mGy (D1)and 1.5 Gy (D2)or 75 mGy (D1)and 25-200 mGy (D2)were invaribly lower than those in the D2 group, while the cell percentages of S phase increased significantly (P<0.05, P<0.01 or P<0.001).Conclusion The results suggest that EL-4 lymphoma cells were irradiated with 25-100 mGy at 6h before 1.0-2.0 Gy exposure.The low-dose radiation could induce the adaptive response of the cell cycle progression in vitro.
|
|
Received: 12 October 2007
|
|
|
|
|
|
|
|
|
