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| The Primary Study of the radiosensitive effect on the MCF7 Cell Line by Human Lactotransferrin High Expression In vitro |
| WANG Yong, YU Xiao-ming, DU Li-qing, et al |
| Institute of Radiation Medicine, Chinese Academy of Medical Sciences, Tianjin 300192 China |
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Abstract Objective To investigate the possible radiosensitive effect of human Lactotransferrin (hLF) high expression in breast cancer cell line MCF7,we hereby constructed and transfected a recombinant vector pBC1 containing exogenous hLF gene.Methods Firstly the recombinant plasmid pBC1-hLF is transferred into MCF7 and the hLF expression was evaluated by western blotting.Then we test the cell viability,apoptosis and clone formation after radiation along with the standard hLF treatment control.Results The hLF gene had been successfully transfect into MCF7 cells and hLF high expression can reduce the clone formation after radiation,and inhibit the cell proliferation with induced apoptosis.But treatment with standard hLF outside of the cell does not work.Conclusions Our primary data shows that hLF high expression can radio sensitize the MCF7 cell in vitro while outside treatment have no such effect.
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Received: 28 June 2009
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