|
|
|
| Improvement of Method for the Technology of Premature Chromosome Condensation in Nuclear Accident |
| GUO Yi-cao, ZHENG Qiao-ling, LI Xi-na, et al |
| Guangdong Prevention and Treatment Center for Occupational Diseases, Guangzhou 510300 China |
|
|
|
|
Abstract Objective To study the improvement of method for the technology of premature chromosome condensation (PCC),and provide a good specimen for quickly estimating radiation dose in nuclear and ionizing accident emergency. Methods The conventional PCC method was used to study the morphology and length of PCC with chemical inducer and mitosis blocker,calyculin A(CA) and colchicines. Improved the dialysis method for harvesting a good morphology of PCC. 2 kinds of cell proliferation promoting agents were added after high dose ionizing radiation with 60Co γ-ray,amygdalin and Cyt-B, to promote the proliferation effects of human peripheral blood lymphocytes. Results ① CA(35 nmol/L) and colchicines(30 nmol/L) were added and cultivated for 50 hours. The PCC with best morphology and structure could be harvested. The cell rate of suitable length vs width was highest(47.4%). ② The dose of hypotonic solution was increased,cultivated in 37℃ water bath and the ratio of fixing solution was changed. The PCC with good specimen could be harvested. ③After being irradiated by 0~20.0 Gy 60Co γ-ray, and the cells were cultivated for 50 hours with amygdalin(150 mg/L) and Cyt-B(0.2 mg/L). The cell proliferation rate was the highest, while the rate in conventional method was lowest. Conclusion The improvement of method could rapidly harvest the good specimen of PCC,it is sutiable for quick evaluation of biological dose when ionizing radiation accident occurred.
|
|
Received: 18 April 2011
|
|
|
|
|
|
|
|
|
