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| Effects of Nimotuzumab Combined with γ-ray Radiation on Growth of the Esophageal Carcinoma Cells |
| ZHANG Rong-xin1, CAO Yong-zhen1, LI Jin2, et al |
1. Department of Radiation Oncology, General Hospital of Tianjin Medical University, Tianjin 300052, China;
2. Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192, China |
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Abstract Objective To study the effect of inhibition of Nimotuzumab(h-R3) and ionizing radiation on the growth of human esophageal carcinoma cells. Methods Human esophageal carcinoma cells were treated with h-R3 or irradiation and the both, respectively. The cell growth inhibition was assessed by MTT. Cell cycle and apoptosis were analyzed by FACS. Results The growth of Ec109 cells treated with h-R3、radiation and h-R3 combined with irradiation were all inhibited. The inhibition rate was concentration-dependent and time-dependent. The group of h-R3 combined with irradiation resulted in greater inhibiting rate(43.74%), compared with h-R3 group and radiation group(P < 0.05). FACS revealed that combined treatment with h-R3 and radiation could not only increase cell cycle(G2 phases) arrest but also cell apoptosis of Ec109 cells. Cells of G2(31.67%) and apoptosis rate(23.62%) for h-R3 combined with radiation group were the highest. Conclusion h-R3 significantly inhibits the proliferation of Ec109 cells in vitro. Combined treatment with h-R3 and radiation shows synergism for the inhibition of esophagus carcinoma cells growth.
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Received: 19 February 2011
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