|
|
|
| Studies on the Mechanism of Apoptasis by Radon in Lung Tissue of Mice |
| PIAO Chun-nan, TIAN Mei, LIU Jian-xiong, et al |
| National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088 China |
|
|
|
|
Abstract Objective To study the mechanism of apoptasis by radon in lung tissue of mice.Methods Male BALB/c mice were exposed to radon with the cumulative dose of 0.02,30 or 60 working level month (WLM) respectively,and then were raised for different time (24h、30d or 90d).Apoptosis was detected by terminal deoxynucleotidy transferase-mediated dUTP-biotin nick end labeling (TUNEL).The expression of p53 and Bcl-2/Bax protein was observed by immunohistochemistry.Results As compared with the control group,the apoptotic index in lung tissue increased along with the increasing of expose dose and the raise time.The protein expression of p53 increased significantlyin the 30d and 90d groups.But Bcl-2/Bax expression decreased.Conclusions The apoptosis by radon in lung tissue of mice had close relationship with p53 and.Bcl-2/Bax pathway.
|
|
Received: 02 December 2010
|
|
|
|
|
|
|
|
|
