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| Comparison of Radiosensitivity of Immune Cells in Three Kinds of Mice |
| WU Hong-ying, WANG Yue-ying, ZHANG Heng, et al |
| Tianjin Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Science, Tianjin 300192 China |
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Abstract Objective To compar the radiosensitivity of thymus (Th), spleen (Sp) cells between IRM-2, ICR and 615 mouse and explor the immunological and radiation resistance mechanisms of IRM-2 mice.Methods The peripheral blood cytotype was determined by flow cytometry.By PA Law (FITC-Annexin V and PI labeling), the apoptosis of Th and Sp cells was detected after irradiation.Results CD4/CD8 ratio of IRM-2 mice was lower than that of ICR and 615 mice;CD25/CD4 ratio was higher than 615 mice (P<0.05), lower than ICR mice;apoptosis rate of Sp cells exposed to 0、1、4 Gy γ-ray was lower than ICR and 615 mice, the difference was of statistical significance (P<0.05);apoptosis rate of Th cell was lower than ICR and 615 mice, especially 615 mice in 4Gy irradiated group (P<0.01).Conclusion The apoptosis rate of IRM-2 was lower than that of ICR and 615 mice after irradiation.Th and Sp cells of IRM-2 mice were not sensitive to radiation.
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Received: 13 October 2009
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