|
|
|
| A Study of the Expression of Bcl-2 and Bax in Rat Hearts Induced by Electromagnetic Radiations |
| DOU Yan1, WANG De-wen2, ZHANG Min-feng1, et al |
1. The 175th hospital of PLA, Zhangzhou 363000 China;
2. Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850 China |
|
|
|
|
Abstract Objective To study contrastly the variation of the expression of Bcl-2 and Bax protein in rat hearts induced by three kinds of Band Electromagnetic Radiations [X-band、S-band and Electromagnetic pluse (EMP)]and investigate the possible mechanism of apoptosis.Methods 180 healthy Wistar rats were randomly divided into four groups:a control group (n=36) and three experimental groups (n=48) respectively treated with X-band、S-band high power microwave and EMP.At different time points (6 hours, 1、3、7、14、28 days, 6 months, 12 months), the rats were killed after irradiated.We observed the pathological changes, examined the expression of Bcl-2 and Bax protein by using immunohistochemical technique and study the rule of change quantitatively by using imagine analysis technique.Results The injury of rat hearts was more and more serious at 6 hours-7 days:cardiomyofilaments were deranged;Glycogen were dicreased;Purkinje cells lysis;martix swelling and exudative serous.The expression of Bax and Bcl-2 protein was both stronger than the control group's (P<0.05).It means apotosis increase.The injured hearts showed resumptive at 14-28 days, and returned to normal progressively at 6-12 months.Conclusion Apoptosis might play an important role in the process of hearts injury induced by electromagnetic radiations and was relative to the expression of Bcl-2 and Bax protein.The injury grades are X > S > EMP.
|
|
Received: 21 January 2010
|
|
|
|
|
|
|
|
|
