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| RNAi-Mediated Rad51 Gene Silenceing Enhances Sensitivity to γ-radiation in Osteosarcoma Cell Lines |
| DU Li-qing1, BAI Jian-qiang2, CAO Jia1, et al |
| Institute of Radiation Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300192 China |
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Abstract Objective To investigate the effects of Rad51 depletion on sensitization to γ-radiation in osteosarcoma cell lines.Methods Four human osteosarcoma cells were examined for basal levels of Rad51 by western blotting analysis and RT-PCR.The suppression of Rad51 expression was performed using vector-based shRNA in HOS cells.Sensitivity of HOS cells treated with shRNA vector to γ-radiation was determined with colony formation assay and MTT assay.The apoptosis was determined by Flow cytometry.Results Rad51 showed higher basal levels in osteosarcoma cell lines than those in CCC-ESF-1 both in protein and RNA level.The suppression of Rad51 expression sensitized apoptosis of the cancer cells induced by γ-radiation.Conclusion Rad51 may serve as a promising therapeutic target for osteosarcoma.
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Received: 15 July 2009
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