补体在放射性肺损伤中的作用与机制

doi:10.13491/j.issn.1004-714X.2022.05.002

中国辐射卫生

2022, Vol. 31

Issue (5): 535-541 DOI: 10.13491/j.issn.1004-714X.2022.05.002

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补体在放射性肺损伤中的作用与机制

耿爽¹, 李倩^1,2, 郗停停¹, 王美玉^1,3, 郭浩鑫^1,2, 王志鑫¹, 毕晓光¹, 鄢成名¹, 杨陟华¹, 王易龙¹, 朱茂祥^1,2,3

1. 军事科学院军事医学研究院辐射医学研究所，北京市放射生物学重点实验室，北京 100850;
2. 南华大学，湖南衡阳 421001;
3. 河北大学生命科学学院，河北保定 071002

Role of complement in radiation-induced lung injury

GENG Shuang¹, LI Qian^1,2, XI Tingting¹, WANG Meiyu^1,3, GUO Haoxin^1,2, WANG Zhixin¹, BI Xiaoguang¹, YAN Chengming¹, YANG Zhihua¹, WANG Yilong¹, ZHU Maoxiang^1,2,3

1. Institute of Radiation Medicine, Academy of Military Medical Sciences, Academy of Military Sciences, Beijing Key Laboratory for Radiobiology, Beijing 100850 China;
2. Nanhua University, Hengyang 421001 China;
3. College of Life Sciences, Hebei University, Baoding 071002 China

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摘要目的探明补体在⁶⁰Co γ射线单次20 Gy胸部照射所致小鼠放射性肺损伤中的作用。方法 ⁶⁰Co γ射线单次20 Gy胸部照射C57BL/6小鼠，观察早期（15 d内）肺组织炎性反应和后期（30 d、180 d）肺纤维化，ELISA测定照射后1 d、3 d、7 d、15 d、30 d、180 d肺组织C2、C3a、C4、C5b-9含量，RT-PCR检测Beas-2B细胞照射后补体mRNA的表达。结果照射后小鼠放射性肺损伤表现为早期炎性反应和晚期纤维化。补体C2、C4和C5b-9 复合物在照射后早期（3 d或7 d）增高（P < 0.05），可能与照射引起的炎性反应有关。补体C3a在照射后3～180 d均明显高于对照组水平，提示其与放射性肺损伤关系密切。照射细胞中，补体C2、C3的mRNA表达增高，而C4、C5的mRNA水平无变化。结论不同补体在放射性肺损伤中的反应存在差异，其中补体C3a与放射性肺损伤关系极为密切，提示通过调控补体C3a及其受体可能是防治放射性肺损伤的新途径。

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	耿爽
	李倩
	郗停停
	王美玉
	郭浩鑫
	王志鑫
	毕晓光
	鄢成名
	杨陟华
	王易龙
	朱茂祥

关键词 ：补体, ⁶⁰Co γ射线, 放射性肺损伤

收稿日期: 2022-04-12

PACS:

Q691

通讯作者: 朱茂祥，E-mail：zhumx2016@126.com E-mail: zhumx2016@126.com

作者简介: 耿爽（1991—），男，河北保定人，硕士研究生，从事放射性肺损伤研究。E-mail：geng0107@126.com

引用本文:

耿爽, 李倩, 郗停停, 王美玉, 郭浩鑫, 王志鑫, 毕晓光, 鄢成名, 杨陟华, 王易龙, 朱茂祥. 补体在放射性肺损伤中的作用与机制[J]. 中国辐射卫生, 2022, 31(5): 535-541.
GENG Shuang, LI Qian, XI Tingting, WANG Meiyu, GUO Haoxin, WANG Zhixin, BI Xiaoguang, YAN Chengming, YANG Zhihua, WANG Yilong, ZHU Maoxiang. Role of complement in radiation-induced lung injury. Chinese Journal of Radiological Health, 2022, 31(5): 535-541.

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