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| Antigen presentation and T cell activation by dendritic cells in radiation damage |
| LI Qian1, GENG Shuang2, YAN Chengming2, GUO Haoxin1, WANG Zhixin2, WANG Meiyu2,3, LIU Benbo2, WANG Xu2, WANG Yilong2, YANG Zhihua2, ZHU Maoxiang1,2,3 |
1. School of Public Health, University of South China, Hengyang 421001 China;
2. Institute of Radiation Medicine, Academy of Military Medicine, Academy of Military Sciences, Beijing Key Laboratory of Radiobiology, Beijing 100850 China;
3. College of Life Sciences, Hebei University, Baoding 071002 China |
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Abstract Objective To explore dendritic cells (DCs)-mediated antigen presentation for radiation-injured cells by using the in vitro cell co-culture technology to simulate the in vivo microenvironment of the lung tissue. Methods 60Co γ-irradiated mouse lung epithelial cells (MLE-12) were cultured with bone marrow-derived DCs and/or splenic T lymphocytes for 48 hours. Flow cytometry was used to measure the expression levels of costimulatory molecules (CD80/86) and antigenic peptide recognition complexes (the major histocompatibility complex [MHC] class Ⅰ/Ⅱ) on DCs and T cell activation markers (CD69/28/152) as well as the numbers of CD4+ and CD8+ T cells.Results 60Co γ irradiation significantly increased the apoptosis rate of MLE-12 cells in a dose-dependent manner, and significantly stimulated the expression of CD80/86 and MHC Ⅱ on DCs, without direct activation of T cells. After γ (6 Gy)-irradiated MLE-12 cells were co-cultured with DCs and T lymphocytes for 48 h, there were significant increases in the expression of CD69 and CD28 on T cells, the numbers of CD4+ and CD8+ T cells, and the expression of CD86 and MHC I on DCs, as compared with the control groups. Conclusion Radiation-injured cells can stimulate antigen presentation by DCs and activate T cells.
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Received: 10 March 2022
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