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| A Method for Isolating、Identifying and Culturing of Rat Trachea-bronchia Epithelial Cells |
| CUI Feng-mei, SU Shi-biao, NIE Jihua, et al |
| School of Radiation Medicine and Public Health, Soochow University, Suzhou 215007, China |
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Abstract Objective To explore a method for isolating、identifying and culturing the rat trachea-bronchia epithelial cells. Methods The rat trachea-bronchia epithelial cells were isolated by digestion with pronase and brushing with cell brush, identified using confocul and cultured in entire F12 media with no serum.Results With this method, cells in high purity and high viability could be obtained, and about 106 cells per rat. The cells grow well in entire F12 media with no serum.Conclusion The method is useful for isolating rat trachea-bronchia epithelial cells and the entire F12 media with no serum is effective for culturing.
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Received: 10 March 2005
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