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| Effect of Eucheuma Gelatinae Polysaccharide on Cycle of Thmocytes and Cell Proliferation in γ-rays Irradinated Mice |
| WU Xianjing1, MENG Qing-yong2 |
| Affiliated Hospital of Guangdong Medical College.Zhanjiang 524001 China |
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Abstract Objective To study the effect of γ-irradiation on cycle of thymocytes cell and proliferation in mice and the radioprotective effect of Eucheuma gelatinae polysaccharide (EGP)in term of cellular level. Methods The mice were randomly divided into 5 groups, the normal group, the model group and 3 EGP treatment groups. EGP of three different doses was applied to different groups respectively for 10 days before whole body irradiation with γ-rays (2.0Gy). The percentage of cell cycle was tested by flowcytometry after 20 h, spontaneous incorporation of thymocytes and proliferation response of splenocytes to ConA and LPS were examined. Results The percentage of thmocytes increased markedly in G0/G1 phase (P <0.01), the percentage of its decreased in s and (M + G2)phase (P <0.01), compared with normal group after γ-rays (2.0 Gy). EGP raised S、G2 +M phase after irradiated mice compared with single irradiated group (P <0.01); EGP could enhance spontaneous incurporation of 3 H-TdR into the mocytes, the proliferation response of splenocytes to ConA and LPS were markedly accelerated in the radiation mice treated EGP, compared with model group. Conclusion EGP has the protectire role in cell radiation injury.
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Received: 17 May 2005
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