BMSC促进巨噬细胞M2型极化减轻急性放射性肺损伤

doi:10.13491/j.issn.1004-714X.2024.01.004

中国辐射卫生

2024, Vol. 33

Issue (1): 21-27 DOI: 10.13491/j.issn.1004-714X.2024.01.004

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BMSC促进巨噬细胞M2型极化减轻急性放射性肺损伤

张鑫辉^1,2, 牛世英^1,3, 姚树桐^1,2, 张晓月¹, 曹雪涛^1,2, 高雪^1,2, 赵国丽⁴, 陈敬坤², 张月英^1,2

1. 山东第一医科大学附属第一医院病理科, 山东济南 250013;
2. 山东第一医科大学临床与基础医学院病理生理学系, 山东济南 250117;
3. 山西省临汾市中心医院病理科, 山西临汾 041099;
4. 聊城市传染病医院病理科, 山东聊城 252002

BMSCs promote M2 macrophage polarization to attenuate acute radiation-induced lung injury

ZHANG Xinhui^1,2, NIU Shiying^1,3, YAO Shutong^1,2, ZHANG Xiaoyue¹, CAO Xuetao^1,2, GAO Xue^1,2, ZHAO Guoli⁴, CHEN Jingkun², ZHANG Yueying^1,2

1. Department of Pathology, the First Affiliated Hospital of Shandong First Medical University, Jinan 250013 China;
2. Department of Pathophysiology, School of Clinical and Basic Medicine, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan 250117 China;
3. Department of Pathology, Linfen Central Hospital, Linfen 041099 China;
4. Department of Pathology, Liaocheng Infectious Disease Hospital, Liaocheng 252002 China

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摘要目的探讨骨髓间充质干细胞(BMSCs)对放射性肺损伤（RILI）的治疗作用，初步探讨其作用机制。方法将45只健康成年雄性C57BL/6小鼠随机分为Control组、Model组、BMSCs组。Model组和BMSCs组单次胸部照射剂量为20 Gy，正常对照组不接受X线照射，照射后6 h内，BMSCs组以1×10⁶ 个BMSCs通过尾静脉注射小鼠体内。第5周取肺组织HE染色观察肺组织病理变化；免疫组织化学染色观察肺组织炎症因子白细胞介素6（IL-6）和肿瘤坏死因子α（TNF-α）的表达变化；巨噬细胞免疫荧光染色观察肺部巨噬细胞极化的变化；免疫蛋白印迹检测肺组织上皮间质转化标志物上皮钙黏着蛋白（E-cadherin）、神经钙黏着蛋白（N-cadherin）、波形蛋白（Vimentin）的表达。结果辐射后，Model组肺组织血管扩张充血，肺间隔明显增厚，炎细胞浸润，BMSCs组小鼠肺组织的上述病变明显减轻；Model组明显增高的在炎症因子IL-6和TNF-α的表达在BMSCs组均明显下调（P < 0.01，P < 0.05)；BMSCs的治疗显著增加肺部巨噬细胞向M2型极化，同时降低了RILI小鼠体内异常增高的N-cadherin和Vimentin的蛋白水平(P < 0.05, P < 0.01)。结论 BMSCs对小鼠RILI有明显的治疗作用，其机制可能与BMSCs促进巨噬细胞M1型向M2型极化有关。

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	张鑫辉
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	姚树桐
	张晓月
	曹雪涛
	高雪
	赵国丽
	陈敬坤
	张月英

关键词 ：放射性肺损伤, 骨髓间充质干细胞, 巨噬细胞极化, 上皮间质转化

收稿日期: 2023-09-12

PACS:

R818

基金资助:山东省自然科学基金（NoZR2020QH208）；山东省医药卫生科技发展计划项目（No202101041037）；山东第一医科大学大学生创新创业训练计划项目（2022104391726）

通讯作者: 张月英,E-mail:zhangyueying828@126.com E-mail: zhangyueying828@126.com

作者简介: 张鑫辉(1998-),男,山东潍坊人,硕士,从事实验病理学的基础研究工作。E-mail:zxh98117@163.com

引用本文:

张鑫辉, 牛世英, 姚树桐, 张晓月, 曹雪涛, 高雪, 赵国丽, 陈敬坤, 张月英. BMSC促进巨噬细胞M2型极化减轻急性放射性肺损伤[J]. 中国辐射卫生, 2024, 33(1): 21-27.
ZHANG Xinhui, NIU Shiying, YAO Shutong, ZHANG Xiaoyue, CAO Xuetao, GAO Xue, ZHAO Guoli, CHEN Jingkun, ZHANG Yueying. BMSCs promote M2 macrophage polarization to attenuate acute radiation-induced lung injury. Chinese Journal of Radiological Health, 2024, 33(1): 21-27.

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http://www.zgfsws.com/CN/10.13491/j.issn.1004-714X.2024.01.004 或 http://www.zgfsws.com/CN/Y2024/V33/I1/21

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