低剂量电离辐射对HBE细胞氧化应激及损伤修复影响

doi:10.13491/j.issn.1004-714X.2023.02.012

中国辐射卫生

2023, Vol. 32

Issue (2): 150-155 DOI: 10.13491/j.issn.1004-714X.2023.02.012

论著

本期目录 | 过刊浏览 | 高级检索

低剂量电离辐射对HBE细胞氧化应激及损伤修复影响

周林倩^1,2, 黄伟旭², 蔡丽娜^2,3, 柯伟奕^2,3, 张灵钰^1,2, 蔡雅诗^2,4, 张素芬², 杨萍¹, 邹剑明², 陈慧峰^1,2

1. 广州医科大学公共卫生学院，广东广州 511436;
2. 广东省职业病防治院，广东省职业病防治重点实验室，广东广州 510300;
3. 广东药科大学公共卫生学院，广东广州 510006;
4. 南方医科大学公共卫生学院，广东广州 510515

Effects of low-dose radiation on oxidative stress and damage repair in HBE cells

ZHOU Linqian^1,2, HUANG Weixu², CAI Lina^2,3, KE Weiyi^2,3, ZHANG Lingyu^1,2, CAI Yashi^2,4, ZHANG Sufen², YANG Ping¹, ZOU Jianming², CHEN Huifeng^1,2

1. School of Public Health, Guangzhou Medical University, Guangzhou 511436 China;
2. Guangdong Key Laboratory of Occupational Disease Prevention and Control, Guangdong Province Hospital for Occupational Disease Prevention and Treatment, Guangzhou 510300 China;
3. School of Public Health, Guangdong Pharmaceutical University, Guangzhou 510006 China;
4. School of Public Health, Southern Medical University, Guangzhou 510515 China

摘要
图/表
参考文献
相关文章 (12)

全文: PDF (745 KB) HTML ( )
输出: BibTeX | EndNote (RIS)

摘要目的探讨低剂量电离辐射（LDIR）对HBE细胞氧化应激及损伤修复的影响。方法将HBE细胞分为0、50、100、200 mGy组，采用X射线照射后分别培养24 h和48 h，检测细胞存活率、谷胱甘肽（GSH）、丙二醛（MDA）、8-羟基脱氧鸟苷（8-OHdG）以及DNA损伤修复相关基因PPP2R2D和TP53基因转录水平。结果与对照组相比，辐照后24 h，各剂量组细胞存活率差异无统计学意义（P > 0.05）；各剂量组MDA水平均显著升高，GSH水平呈剂量依赖性降低，8-OHdG水平呈剂量依赖性升高，PPP2R2D基因mRNA水平均显著升高，差异具有统计学意义（P < 0.05）；50 mGy组TP53基因mRNA表达水平显著升高，差异具有统计学意义（P < 0.05）。与对照组相比，辐照后48 h，50 mGy组细胞存活率达到最高，MDA水平显著降低，8-OHdG水平显著降低，PPP2R2D基因和TP53基因mRNA表达水平均显著升高，差异具有统计学意义（P < 0.05）；100 mGy组细胞GSH水平显著升高，差异具有统计学意义（P < 0.05）。结论 LDIR特别是50 mGy的辐射可影响HBE细胞氧化-抗氧化水平以及DNA损伤修复相关基因转录水平差异表达。

	服务

	把本文推荐给朋友
	加入我的书架
	加入引用管理器
	E-mail Alert
	RSS
	作者相关文章
	周林倩
	黄伟旭
	蔡丽娜
	柯伟奕
	张灵钰
	蔡雅诗
	张素芬
	杨萍
	邹剑明
	陈慧峰

关键词 ：低剂量电离辐射, 氧化应激, DNA氧化损伤, DNA修复

收稿日期: 2022-09-23

PACS:

X591

基金资助:国家自然科学基金（81302387）；广东省职业病防治重点实验室（2017B030314152）；广东省基础与应用基础研究基金（2019A1515011969、2022A1515012421、2023A1515010414）；广州市科技计划项目（202002030031）；广东省医学科学技术研究基金（B2021386）

通讯作者: 陈慧峰,E-mail:hfchen2001@163.com E-mail: hfchen2001@163.com

作者简介: 周林倩（1997—），女，布依族，贵州三都人，在读硕士研究生，从事低剂量电离辐射生物学效应研究。E-mail：850497101@qq.com

引用本文:

周林倩, 黄伟旭, 蔡丽娜, 柯伟奕, 张灵钰, 蔡雅诗, 张素芬, 杨萍, 邹剑明, 陈慧峰. 低剂量电离辐射对HBE细胞氧化应激及损伤修复影响[J]. 中国辐射卫生, 2023, 32(2): 150-155.
ZHOU Linqian, HUANG Weixu, CAI Lina, KE Weiyi, ZHANG Lingyu, CAI Yashi, ZHANG Sufen, YANG Ping, ZOU Jianming, CHEN Huifeng. Effects of low-dose radiation on oxidative stress and damage repair in HBE cells. Chinese Journal of Radiological Health, 2023, 32(2): 150-155.

链接本文:

http://www.zgfsws.com/CN/10.13491/j.issn.1004-714X.2023.02.012 或 http://www.zgfsws.com/CN/Y2023/V32/I2/150

[1] United Nations Scientific Committee on the Effects of Atomic Radiation. Report of the United Nations Scientific Committee on the Effects of Atomic Radiation 2010[Z]. New York: United Nations, 2011.
[2] 陈慧峰, 郭强之, 刘明, 等. 广东高本底地区人群氧化损伤及抗氧化水平调查[J]. 中华放射医学与防护杂志,2015,35(2):83-87. DOI: 10.3760/cma.j.issn.0254-5098.2015.02.002
Chen HF, Guo QZ, Liu M, et al. The effects of low dose radiation on the levels of oxidative damage and antioxidant in population of high background radiation area of Guangdong[J]. Chin J Radiol Med Prot, 2015, 35(2): 83-87. DOI: 10.3760/cma.j.issn.0254-5098.2015.02.002
[3] Bankoglu EE, Gerber J, Kodandaraman G, et al. Influence of bariatric surgery induced weight loss on oxidative DNA damage[J]. Mutat Res Genet Toxicol Environ Mutagen, 2020, 853: 503194. DOI: 10.1016/j.mrgentox.2020.503194
[4] Ghahe SS, Kosicki K, Wojewódzka M, et al. Increased DNA repair capacity augments resistance of glioblastoma cells to photodynamic therapy[J]. DNA Repair (Amst), 2021, 104: 103136. DOI: 10.1016/j.dnarep.2021.103136
[5] 赵文迪, 田健, 周建炜. 低剂量/低剂量率电离辐射减轻放射性损伤的研究进展[J]. 河南大学学报(医学版),2021,40(5):372-376. DOI: 10.15991/j.cnki.41-1361/r.2021.05.012
Zhao WD, Tian J, Zhou JW. Research progress of low-dose/low-dose-rate ionizing radiation in reducing radiation damage[J]. J Henan Univ (Med Sci), 2021, 40(5): 372-376. DOI: 10.15991/j.cnki.41-1361/r.2021.05.012
[6] Harris IS, DeNicola GM. The complex interplay between antioxidants and ROS in cancer[J]. Trends Cell Biol, 2020, 30(6): 440-451. DOI: 10.1016/j.tcb.2020.03.002
[7] Lei G, Zhang YL, Koppula P, et al. The role of ferroptosis in ionizing radiation-induced cell death and tumor suppression[J]. Cell Res, 2020, 30(2): 146-162. DOI: 10.1038/s41422-019-0263-3
[8] 常馨月, 米晓, 董明然, 等. 巴西海木提取物抗生殖系统氧化损伤活性研究[J]. 中南药学,2022,20(4):751-758. DOI: 10.7539/j.issn.1672-2981.2022.04.005
Chang XY, Mi X, Dong MR, et al. Antioxidant activity of Trichilia catigua A. Juss. against damage in the reproductive system[J]. Central South Pharm, 2022, 20(4): 751-758. DOI: 10.7539/j.issn.1672-2981.2022.04.005
[9] 杨柳春, 王克义. DNA氧化损伤标志物8-羟基脱氧鸟苷与相关肿瘤关系的研究进展[J]. 浙江医学,2021,43(23):2608-2612. DOI: 10.12056/j.issn.1006-2785.2021.43.23.2021-3119
Yang LC, Wang KY. Advances in the relationship between 8-hydroxydeoxyguanosine, a marker of oxidative DNA damage, and related tumours[J]. Zhejiang Med J, 2021, 43(23): 2608-2612. DOI: 10.12056/j.issn.1006-2785.2021.43.23.2021-3119
[10] Fijen C, Rothenberg E. The evolving complexity of DNA damage foci: RNA, condensates and chromatin in DNA double-strand break repair[J]. DNA Repair (Amst), 2021, 105: 103170. DOI: 10.1016/j.dnarep.2021.103170
[11] 余小玲, 荣利, 方芳, 等. 低剂量X线辐射对A549细胞凋亡的适应性反应[J]. 中国辐射卫生,2022,31(2):139-143,148. DOI: 10.13491/j.issn.1004-714X.2022.02.001
Yu XL, Rong L, Fang F, et al. Adaptive response of A549 cell apoptosis induced by low-dose X-ray irradiation[J]. Chin J Radiol Health, 2022, 31(2): 139-143,148. DOI: 10.13491/j.issn.1004-714X.2022.02.001
[12] Boutelle AM, Attardi LD. p53 and tumor suppression: it takes a network[J]. Trends Cell Biol, 2021, 31(4): 298-310. DOI: 10.1016/j.tcb.2020.12.011
[13] Mumby M. PP2A: unveiling a reluctant tumor suppressor[J]. Cell, 2007, 130(1): 21-24. DOI: 10.1016/j.cell.2007.06.034
[14] Lambrecht C, Haesen D, Sents W, et al. Structure, regulation, and pharmacological modulation of PP2A phosphatases[M]//Millán JL. Phosphatase Modulators. Totowa: Humana Press, 2013: 283-305. DOI: 10.1007/978-1-62703-562-0_17.
[15] 陈慧峰, 谭斯文, 闫雪华, 等. 不同剂量电离辐射对人外周血淋巴细胞DNA损伤修复相关基因转录水平的影响[J]. 中国辐射卫生,2020,29(1):7-12. DOI: 10.13491/j.issn.1004-714X.2020.01.002
Chen HF, Tan SW, Yan XH, et al. Effects of different dose ionizing radiation on the transcriptional level of DNA damage repair related genes in human peripheral blood lymphocytes[J]. Chin J Radiol Health, 2020, 29(1): 7-12. DOI: 10.13491/j.issn.1004-714X.2020.01.002
[16] Kim SY, Hyun SY, Jang YJ. Dephosphorylation of Plk1 occurs through PP2A-B55/ENSA/Greatwall pathway during mitotic DNA damage recovery[J]. Cell Cycle, 2019, 18(10): 1154-1167. DOI: 10.1080/15384101.2019.1617003
[17] 王茂枝, 刘森林. 辐射的来源漫谈[J]. 中国辐射卫生,2021,30(2):238-243. DOI: 10.13491/j.issn.1004-714X.2021.02.025
Wang MZ, Liu SL. The sources of radiation[J]. Chin J Radiol Health, 2021, 30(2): 238-243. DOI: 10.13491/j.issn.1004-714X.2021.02.025
[18] 唐颖. 阳江高本底辐射地区居民IL-2、IL-2R基因表达水平和IL-2R基因多态性研究[D]. 广州: 广东药科大学, 2020. DOI: 10.27690/d.cnki.ggdyk.2020.000258.
Tang Y. Study on IL-2, IL-2R gene expression level and IL-2R gene polymorphism in Yangjiang high background radiation area[D]. Guangzhou: Guangdong Pharmaceutical University, 2020. DOI: 10.27690/d.cnki.ggdyk.2020.000258.
[19] 康爽, 谭业辉, 郎悦. 低剂量辐射对人骨髓间充质干细胞的兴奋效应[J]. 中国老年学杂志,2015,35(9):2586-2588. DOI: 10.3969/j.issn.1005-9202.2015.09.133
Kang S, Tan YH, Lang Y. Excitatory effects of low-dose radiation on human bone marrow mesenchymal stem cells[J]. Chin J Gerontol, 2015, 35(9): 2586-2588. DOI: 10.3969/j.issn.1005-9202.2015.09.133
[20] Konkova M, Abramova M, Kalianov A, et al. Mesenchymal stem cells early response to low-dose ionizing radiation[J]. Front Cell Dev Biol, 2020, 8: 584497. DOI: 10.3389/fcell.2020.584497
[21] 杨国姿. ATM相关信号通路在低剂量辐射诱导肺正常及肿瘤细胞生物学效应差异中的作用及机制研究[D]. 长春: 吉林大学, 2016.
Yang GZ. Distinct biological effects of low-dose radiation on lung normal and cancerous cells are mediated by ATM signaling[D]. Changchun: Jilin University, 2016.
[22] 王琪, 赵珂, 高雅萌, 等. 电离辐射对小鼠造血干/祖细胞线粒体功能的影响[J]. 中华放射医学与防护杂志,2022,42(5):321-327. DOI: 10.3760/cma.j.cn112271-20220222-00064
Wang Q, Zhao K, Gao YM, et al. Effects of ionizing radiation on mitochondrial function of mouse hematopoietic stem and progenitor cells[J]. Chin J Radiol Med Prot, 2022, 42(5): 321-327. DOI: 10.3760/cma.j.cn112271-20220222-00064
[23] Tabatabaie F, Franich R, Feltis B, et al. Oxidative damage to mitochondria enhanced by ionising radiation and gold nanoparticles in cancer cells[J]. Int J Mol Sci, 2022, 23(13): 6887. DOI: 10.3390/ijms23136887
[24] 胡昌坤, 张雪梅, 马增春, 等. 低剂量辐射对小鼠肠道损伤的影响[J]. 辐射防护,2021,41(4):315-320
Hu CK, Zhang XM, Ma ZC, et al. Effects of low dose radiation on intestinal injury in mice[J]. Radiat Prot, 2021, 41(4): 315-320
[25] Winterbourn CC, Hampton MB. Thiol chemistry and specificity in redox signaling[J]. Free Radic Biol Med, 2008, 45(5): 549-561. DOI: 10.1016/j.freeradbiomed.2008.05.004
[26] 于卫国, 张杰, 张蕾, 等. 丹参对电离辐射致SD大鼠损伤的防护研究[J]. 江西医药,2019,54(10):1193-1195. DOI: 10.3969/j.issn.1006-2238.2019.10.014
Yu WG, Zhang J, Zhang L, et al. Protective effects of Danshen on SD rats induced by ionizing radiation[J]. Jiangxi Med J, 2019, 54(10): 1193-1195. DOI: 10.3969/j.issn.1006-2238.2019.10.014
[27] Jelic MD, Mandic AD, Maricic SM, et al. Oxidative stress and its role in cancer[J]. J Cancer Res Ther, 2021, 17(1): 22-28. DOI: 10.4103/jcrt.JCRT_862_16
[28] 刘明, 苏世标, 郭强之, 等. 高本底地区辐射对8-OHdG和hMTH1的影响研究[J]. 中国热带医学,2016,16(7):670-672. DOI: 10.13604/j.cnki.46-1064/r.2016.07.11
Liu M, Su SB, Guo QZ, et al. Impact of radiation on 8-OHd G and h MTH1 in high background radiation area[J]. China Trop Med, 2016, 16(7): 670-672. DOI: 10.13604/j.cnki.46-1064/r.2016.07.11
[29] Dung TD, Day CH, Binh TV, et al. PP2A mediates diosmin p53 activation to block HA22T cell proliferation and tumor growth in xenografted nude mice through PI3K-Akt-MDM2 signaling suppression[J]. Food Chem Toxicol, 2012, 50(5): 1802-1810. DOI: 10.1016/j.fct.2012.01.021