外周血淋巴细胞染色体、微核和转化率检查制片技术探讨

doi:10.13491/j.issn.1004-714X.2021.06.004

中国辐射卫生

2021, Vol. 30

Issue (6): 677-681 DOI: 10.13491/j.issn.1004-714X.2021.06.004

辐射健康/论著

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外周血淋巴细胞染色体、微核和转化率检查制片技术探讨

凌攀, 何玲, 王晓凤, 赵芹虹, 赵仪, 陈曦阳

四川省疾病预防控制中心, 四川成都 610041

Preparation technique for the study of chromosome, micronucleus, and transformation rate of peripheral blood lymphocytes

LING Pan, HE Ling, WANG Xiaofeng, ZHAO Qinhong, ZHAO Yi, CHEN Xiyang

Sichuan Center for Diseases Control and Prevention, Chengdu 610041 China

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摘要目的探索淋巴细胞检查的制片技术，提高检查质量和效率。方法以某一无放射接触史者外周静脉血为对照组，以同一人外周静脉血通过剂量率为1.002 Gy/min、剂量为2.8 Gy的⁶⁰Coγ射线辐照后为试验组。37℃孵箱培养淋巴细胞48 h后加入秋水仙素，再培养20 h；将培养液分成低渗与非低渗，预固定，再将二者混合、提纯、制片、染色、分析。用相同标本行标准方法实验。结果改进方法与标准方法比较，试验组的染色体异常率分别为75.8%和74.0%，对照组的染色体异常率均为0。试验组的微核细胞率分别为7.8‰和7.2‰，对照组的微核细胞率分别为1.2‰和1.4‰；试验组的淋巴细胞转化率分别为43.6%和43.2%，对照组的淋巴细胞转化率分别为65.4%和66.2%。结论改进方法和标准方法对外周血淋巴细胞染色体畸变率、微核细胞率和淋巴细胞转化率检测无统计学差异，而改进方法使制片时程缩短，同时也节约了试剂耗材，提高了制片质量，检测项目在一张玻片上完成，缩短了观察时间，提高了报告效率。

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关键词 ：淋巴细胞, 制片, 方法改进

收稿日期: 2021-05-24

PACS:

Q691

通讯作者: 何玲,E-mail:546090651@qq.com.com E-mail: 546090651@qq.com.com

作者简介: 凌攀(1983-),女,四川人,副主任技师,从事医学检验工作,E-mail:43287772@qq.com

引用本文:

凌攀, 何玲, 王晓凤, 赵芹虹, 赵仪, 陈曦阳. 外周血淋巴细胞染色体、微核和转化率检查制片技术探讨[J]. 中国辐射卫生, 2021, 30(6): 677-681.
LING Pan, HE Ling, WANG Xiaofeng, ZHAO Qinhong, ZHAO Yi, CHEN Xiyang. Preparation technique for the study of chromosome, micronucleus, and transformation rate of peripheral blood lymphocytes. , 2021, 30(6): 677-681.

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http://www.zgfsws.com/CN/10.13491/j.issn.1004-714X.2021.06.004 或 http://www.zgfsws.com/CN/Y2021/V30/I6/677

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