小剂量γ射线累积照射对大鼠尿液中小分子代谢物影响

doi:10.13491/j.issn.1004-714x.2018.05.004

中国辐射卫生

2018, Vol. 27

Issue (5): 437-441 DOI: 10.13491/j.issn.1004-714x.2018.05.004

放射生物学/论著

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小剂量γ射线累积照射对大鼠尿液中小分子代谢物影响

张慧芳^1,2, 任越², 王宏力², 胡波², 张忠新², 王超², 郭月凤²

1. 中国原子能科学研究院, 北京 102413;
2. 中国辐射防护研究院

Effects of small dose accumulative γ-irradiation on the small molecular metabolites in rats urine

ZHANG Huifang^1,2, REN Yue², WANG Hongli², HU bo², ZHANG Zhongxin², WANG Chao², GUO Yuefeng²

1. China Institute of Atomic Energy, Beijing 102413 China;
2. China Institute for Radiation Protection

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摘要目的探讨小剂量¹³⁷Cs γ射线累积照射对大鼠尿液中小分子代谢物的影响。方法无特定病原体级健康雄性成年SD大鼠40只，随机分为对照组和照射组，每组20只。照射组大鼠采用¹³⁷Cs γ射线照射，吸收剂量率为0.353 mGy/min，每天累积照射0.083 3 Gy，每3天累积剂量为0.25 Gy；对照组大鼠不作任何处理。分别于照射前1 d和照射开始后第3、6、9、12 d收集大鼠尿液，对照组与照射组开始照射后第6 d同步收集尿样。采用核磁共振技术对大鼠尿样进行检测，结合主成分分析法和偏最小二乘法判别法，分析对照组与各剂量组大鼠尿液中小分子代谢物的差异。结果小剂量γ射线累积照射后，各剂量亚组大鼠尿液中小分子代谢物发生了变化，各剂量组共发现了5种共同差异代谢物，分别为糖类、瓜氨酸、牛磺酸、柠檬酸盐和尿嘧啶。4个剂量亚组大鼠尿中5种差异代谢物相对水平分别高于对照组（P<0.05）；其中牛磺酸相对水平与累积剂量呈正相关关系（P<0.05）。结论小剂量γ射线累积照射可造成大鼠尿液中小分子代谢物扰动。

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	张慧芳
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	王宏力
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	张忠新
	王超
	郭月凤

关键词 ：小剂量, 小分子代谢物, 核磁共振, 主成分分析, 偏最小二乘法

收稿日期: 2018-06-16

PACS:

Q691

作者简介: 张慧芳(1980-),女,在读辐射防护及环境保护专业博士研究生,副研究员,主要从事放射医学研究。

引用本文:

张慧芳, 任越, 王宏力, 胡波, 张忠新, 王超, 郭月凤. 小剂量γ射线累积照射对大鼠尿液中小分子代谢物影响[J]. 中国辐射卫生, 2018, 27(5): 437-441.
ZHANG Huifang, REN Yue, WANG Hongli, HU bo, ZHANG Zhongxin, WANG Chao, GUO Yuefeng. Effects of small dose accumulative γ-irradiation on the small molecular metabolites in rats urine. , 2018, 27(5): 437-441.

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http://www.zgfsws.com/CN/10.13491/j.issn.1004-714x.2018.05.004 或 http://www.zgfsws.com/CN/Y2018/V27/I5/437

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