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| Adaptive Response of Spermatogenic Cell Apoptosis and p53 Gene Expression Selectively Induced by Irradiation with Low Dose X-Ray in Mice |
| LIU Guang-wei1, GONG Ping-sheng2, ZHAO Hong-guang1, et al |
| MH Radiobiology Research Unit, School of Public Health, Jilin University, Changchun 130021, China |
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Abstract Objective The adaptive response of spermatogenic cell apoptosis and p53 gene expression induced by whole-body low dose radiation (LDR) with X-rays was studied in male Kunming mice.Methods Different kinds of spermatogenic cells were separated using density gradient centrifugation and their apoptotic percentagcs were analysed using flow cytometry (FCM) stained with propodium iodide.At meantime,p53 protein was measured with immunohistochemical SABC.Results When inductive dose (D1) was 75 mGy 6 h before irradiation with the challenging doses (D2) of 1.0,2.0 or 3.0 Gy,the apoptotic percentages of the spermatogonia and spermatocytes in the D1+D2 groups were declined rapidly as compared with those in the D2 groups,and the apoptotic percentages of spermatids and spermatozoa showed no significant changes.When inductive dose (D1) was 75 mGy 6 h before irradiation with the challenging doses (D2) of 1.0,2.0 or 3.0 Gy,the percentages of p53 protein positive of spermatogonia and spermatocytes in the D1+D2 groups were declined rapidly as compared with those in the D2 groups,and the positive percentages of spermatids and spermatozoa showed no significant changes.Conclusion The adaptive response of apoptosis and p53 protein expression in spermatogonia and spermatocytes could be selectively induced by irradiation with low dose X-ray.The apoptotic adaptive response induced by LDR could closely relate to the D2 doses and interval time between D1 and D2,so we speculated that p53 gene plays a great role in molecular mechanisms of adaptive response on spermatogenic cell apoptosis induced by LDR.
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Received: 26 January 2005
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